[Objectives] To reveal the influence of illumination on the body colour of Prodenia litura (Fabricius) larvae, a new system of evaluating insect body colour was established. [Method] Based on computer vision detection technology, the change in body colour ofP litura larvae over successive days and the effect of illumination on the change in body colour were evaluated by assigning colours a numerical value. [Results] Brightness (B) and colour index (CO decreased gradually of larvae over successive days, while the deviation coefficient (BDV...