期刊论文

Yu, L.-J.

中文

中草药

0253-2670

2012

43

11

2266-2271

湖南省自然科学基金资助项目（11JJ3040） 湖南省大学生科技创新研究项目（DFCXS201003）

本校为第一机构

目的采用ISSR标记技术从分子水平探讨皱边石杉内生真菌资源的遗传多样性,建立皱边石杉内生真菌资源遗传分化指纹图谱,为筛选可产石杉碱甲的目标菌株提供快捷的判别依据。方法以从皱边石杉中分离的100株内生真菌为材料,建立其ISSR优化反应体系并分析其遗传多样性;TLC、HPLC检测发酵产物。结果优化筛选的10条ISSR引物对皱边石杉内生真菌进行遗传多样性分析,共扩增出3 975条清晰条带,多态性条带占100%。遗传相似系数为0.59~0.96,在0.64水平,皱边石杉内生真菌可分为11类?’在0.6t水平,第I类又可分为5个亚类。采用引物UBC868对13号菌株及皱边石杉基因组DNA扩增,在500、200 bp均具有清晰的扩增条带,发...

Objective: To study the genetic diversity of endophytic fungi of Huperzia crispata using ISSR markers at molecular level, to establish the fingerprint for genetic differentiation, and to provide the reference for screening the endophytic fungi strains which could biosynthesize huperzine A (Hup A). Methods: Endophytic fungi (100) isolated from H. crispata were selected as materials, their optimized ISSR reaction systems were established, and the genetic diversity was analyzed. TLC and HPLC were used to detect the fermentation products. Results: Ten ISSR primers were screened to analyze the gene...