期刊论文

Xiong, X.-Y.

中文

中草药

0253-2670

2014

45

12

1743-1746

10.7501/j.issn.0253-2670.2014.12.017

教育部创新团队项目:园艺植物功能成分优异资源高效利用（IRT0963）；

本校为第一机构

目的研究甜茶苷对变形链球菌Streptococcus mutans致龋能力的影响。方法将变形链球菌菌液加入甜茶苷后于恒温厌氧环境下培养,测试其pH值变化、黏附率,运用碳氢化合物法测定其表面疏水率。采用Bradford法以Neson-samogyi分别测量总蛋白的量以及还原糖的量,计算葡萄糖基转移酶(glucosyltransferase,GTF)活力,采用Anihrone法测量水不溶性葡聚糖(water-insoluble glucans,WIG)的量。结果加入甜茶苷的菌液 pH变化值、黏附率、表面疏水率、GTF活力及 WIG的量与对照组之间均有显著性差异(P

Objective: To study the inhibitory effect of rubusoside on the growth of Streptococcus mutans. Methods: S. mutans was added to each group, cultured in anaerobic incubator with constant temperature, and the pH value was measured and compared. Microbial adhesion to hydrocarbons method was used to test the adhesion and cell surface hydrophobicity. Centrifugation was followed, Bradford method and Neson-samogyi method were used to measure the total contents of protein and reducing sugar and to calculate the viability of glucosyltransferase (GTF). Anihrone method was used to measure the contents of ...