Functional identification of plant secondary metabolite biosynthesis gene in Saccharomyces cerevisiae by cell feeding
作者：
刘金凤;黄鹏;卿志星;刘秀斌;柳亦松;...
通讯作者：
Zeng, J.-G.
作者机构：
湖南农业大学园艺园林学院,湖南长沙410128
湖南农业大学兽用中药资源与中兽药创制国家地方联合工程研究中心,湖南长沙410128
湖南中医药大学药学院,湖南长沙,410128
湖南农业大学动物医学院,湖南长沙410128
[刘秀斌; 刘金凤; 唐其; 黄鹏] Horticulture & Landscape College of Hunan Agricultural University, Changsha, 410128, China, National and Local Union Engineering Research Center for the Veterinary Herbal Medicine Resources and Initiative, Hunan Agricultural University, Changsha, 410128, China
通讯机构：
[Zeng, J.-G.] H
Horticulture & Landscape College of Hunan Agricultural UniversityChina
Objective: To screen the most efficient cell feeding way and provide guidance for functional verification of genes in Saccharomyces cerevisiae. Methods: Firstly, the recombinant industrial S. cerevisiae strains containing PsCPR and PsP6H genes were constructed. Then four different cell feeding methods were used. At the end, the downstream product dihydrosanguinarine was detected via UPLC Q-TOF MS/MS. Results: The best method of cell feeding was that the recombinant industrial strain induced by galactose for 31 h and then incubated in the TE sol...
