期刊论文

Cloning and expression analysis of squalene synthase gene in Hedera helix

中文

中草药

0253-2670

2018

49

9

2127-2132

10.7501/j.issn.0253-2670.2018.09.023

中国热带农业科学院基本科研业务费专项资金（1630032018006） 湖南省教育厅科研项目（15A089）

本校为第一机构

目的 克隆获得洋常春藤Hedera helix鲨烯合成酶基因(HhSS) cDNA全长序列并进行生物信息学分析及表达分析.方法 根据洋常春藤转录组数据信息设计引物,通过RACE克隆方法获得HhSS基因序列;采用DNAMAN、PROTPARAM、TMHMM、PSORT、ScanProsite、SOPMA、SWISS-MODEL等生物信息学工具分析序列信息及编码蛋白的理化特性、结构域等特征;通过实时荧光定量PCR (qRT-PCR)技术进行HhSS基因的检测分析.结果 RACE克隆获得HhSS(GenBank登录号KX056078)基因cDNA序列全长1 889 bp,包含一个从248～1 477 bp的完整开放阅读框(ORF)以及247bp的5'非编码区(5'UTR)和412 bp的3'非编码区(3'UTR).该基因编码409个氨基酸,相...

Objective To obtain a full-length cDNA of squalene synthase gene from Hedera helix (HhSS) by cloning technique, and to carry out bioinformatics analysis and expression analysis. Methods Primers were designed based on H. helix transcriptome data, and HhSS was cloned by using RACE technologies. DNAMAN, PROTPARAM, TMHMM, PSORT, ScanProsite, SOPMA, and SWISS-MODEL were used for analysis of sequence and physical and chemical properties and domain of encoded protein. The relative expression of HhSS was detected by qRT-PCR. Results The cDNA sequence o...