Identification and Functional Characterization of a Geraniol Synthase UrGES from Uncaria rhynchophylla

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成果类型：

期刊论文

作者：

Liu, Xinghui;Chen, Wenqiang;Li, Linxuan;Mu, Detian;Wilson, Iain W.;...

通讯作者：

Tang, Q;Pan, LM

作者机构：

[Xiang, Yahui; Liu, Xinghui; Zhu, Lina; Tang, Qi; Mu, Detian; Chen, Wenqiang] Hunan Agr Univ, Coll Hort, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingre, Changsha 410128, Peoples R China.

[Li, Linxuan; Pan, Limei] Natl Ctr TCM Inheritance & Innovat, Guangxi Key Lab High Qual Format & Utilizat Dao di, Guangxi Bot Garden Med Plants, Nanning 530023, Peoples R China.

[Wilson, Iain W.] CSIRO Agr & Food, Canberra, ACT 2601, Australia.

[Huang, Xueshuang] Hunan Univ Med, Hunan Prov Key Lab Synthet Biol Tradit Chinese Med, Huaihua 418000, Peoples R China.

[Qiu, Deyou] Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China.

通讯机构：

[Pan, LM ] N

[Tang, Q ] H

Hunan Agr Univ, Coll Hort, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingre, Changsha 410128, Peoples R China.

Natl Ctr TCM Inheritance & Innovat, Guangxi Key Lab High Qual Format & Utilizat Dao di, Guangxi Bot Garden Med Plants, Nanning 530023, Peoples R China.

语种：

英文

关键词：

bioinformatics analysis;enzymatic assay;terpene synthase;terpenoid indole alkaloids;transient expression

期刊：

Plants-Basel

ISSN：

2223-7747

年：

2025

卷：

期：

DOI：

10.3390/plants14152273

基金类别：

Guangxi Major Science and Technology Project of China; Hunan Agricultural University for Supporting Young Interdisciplinary Scholars [2024XKJC02]; Hunan Provincial Key Laboratory for Synthetic Biology of Traditional Chinese Medicine [HCSW2023-01]; Project of Hunan Natural Science Foundation [2022JJ30305]; Key Scientific Research Fund of Hunan Provincial Education Department [21A0138]; Horticulture's Open program of Hunan Agricultural University [2021YYXK002]; Postgraduate Scientific Research Innovation Project of Hunan Province [CX20220689]; [GuikeAA22096021]

机构署名：

本校为第一且通讯机构

院系归属：

园艺园林学院

摘要：

Uncaria rhynchophylla, a medicinal plant extensively used in traditional Chinese medicine, is an important plant source of terpenoid indole alkaloids (TIAs), but the mechanism of TIA biosynthesis at molecular level remains unclear. Geraniol synthase (GES) serves as a crucial enzyme in catalyzing the formation of geraniol from geranyl pyrophosphate (GPP) in various plants, but the functional characterization of the GES gene in U. rhynchophylla has not been investigated. In this study, a GES was identified and characterized through genome mining and bioinformatic analysis. Functional validation ...

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Identification and Functional Characterization of a Geraniol Synthase UrGES from Uncaria rhynchophylla

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