As novel fluorescent nanomaterials, quantum dots (QDs) have played important roles in many fields, such as chemical analysis, biology sensor, molecular imaging, owing to their excellent optical properties. Studies on the properties of single quantum dots can help to find new experimental phenomenology which can't be found in the ensemble-approach, provide a mentality to improve the properties of quantum dots, contribute to a better application of quantum dots in various fields. In this review, identifications criterion of single quantum dots, p...