In order to search a method to quickly and accurately diagnose canine distemper, reverse transcriptase-polymerase chain reaction was developed in the research. The templates were isolated from the lymphocytes of pet dogs clinically suspected canine distemper. The primers used in the experiment had been diagnose the disease of police dogs successfully before. The specific 287 bp nucleotides band could be seen on the electrophoresis agarose, which showed that canine dist...
