To diagnose the disease of guinea pig from a farm in Changsha Hunan province, one of the pathogenic bacteria had been isolated from died animal. According to clinical observation, labo- ratory detection and animal pathogenicity test, a case of Streptococcus equi subsp. Zooepidemicus in- fection was confirmed. The morphological observation showed that the bacteria was Gram-positive streptococcus and β hemolysis in blood agar culture, its 16S rRNA gene shared highly homologous with Streptococcus equi subsp, zooepidemicus. The same bacteria could be isol...