期刊论文

中文

经济动物学报

1007-7448

2020

24

1

9-16

10.13326/j.jea.2019.1351

31372406:国家自然科学基金 2018JJ2177:湖南省自然科学基金面上项目 SCX1836/201640307323:大学生研究性学习和创新性实验计划 17QN10:湖南农业大学校科学基金项目

本校为第一机构

猪圆环病毒3型（PCV3）是一种在世界范围内分布较广泛的新型猪病毒,其发病机制尚不清楚。为系统分析PCV3 Capsid（Cap）蛋白序列和结构特点,收集Gen Bank登录的来自不同国家和地区的210条PCV3全基因组和Cap蛋白基因序列,筛选获得46株PCV3代表序列;采用生物信息学（Bio Edit和MAGE 6.0等）技术分析PCV3 Cap序列同源性、结构特点、系统进化和热点突变位点情况。结果表明:46株PCV3 Cap序列同源性为97.66%～100%,PCV3 Cap预测的8个B细胞和10个T细胞表位中均存在序列突变,其中B细胞抗原表位E （109～146位氨基酸）和T细胞抗原表位D（72～81位氨基酸）存在的突变位点数最多。研究发现26株属于PCV3a,20...

Porcine circovirus type 3 is a new porcine virus already distributed worldwide,the pathogenesis of PCV3 remains unknown because isolation and continuous passage of PCV3 is failure in cell lines at present.In order to systematically analyze the sequence and structural characteristics of PCV3 Cap,210 PCV3 whole genome and the sequences of cap gene from different countries and regions published in Gen Bank were collected respectively,then 46 PCV3 representative sequences were screened,and the homology,structural features,phyletic evolution and hot spot mutation site of PCV3 Cap sequences were ana...