会议论文

MOLECULAR CLONING AND TISSUE EXPRESSION ANALYSIS OF Mx GENE IN SQUALIOBARBUS CURRICULUS

中文

2017

187-188

中国海洋湖沼学会、中国动物学会鱼类学分会第九届会员代表大会暨2014年学术研讨会

中国海洋湖沼学会、中国动物学会鱼类学分会第九届会员代表大会暨2014年学术研讨会论文集

20140828

天津

中国海洋湖沼学会<&wdkj&>中国动物学会

本校为第一机构

　　为研究赤眼鳟(Squaliobarbus curriculus)Mx蛋白(Myxovirus resistance protein)的功能,采用简并PCR和SMART RACE方法从赤眼鳟脾脏中克隆得到Mx基因全长cDNA,并通过生物信息学方法分析其同源性,再利用实时荧光定量PCR (RT-qPCR)检测其在脾、肝、肠、肾等9个组织中的表达,以及感染草鱼呼肠孤病毒(Reovirus of Grass carp) GCRV-104后不同时间点赤眼鳟Mx的时空表达规律。

　　Mx protein with broad-spectrum antiviral activity is a kind of GTP-enzyme active protein induced by interferon.As a kind of economically fresh water fish with superior quality,Squaliobarbus curriculus has the highest fitness and disease resistance,but few researches have reported in terms of its immunity.In this research,we amplified a full-length cDNA of Mx (ScMx) from the spleen of Squaliobarbus curriculus by using degenerate PCR and SMART RACE,conducted homology analysis and tissue expression analysis of ScMx mRNA in different tissues (l...