To resolve the problem of low survival rate of Dioscorea zingiberensis C.H.Wright plantlets cultured in vitro,a new micropropagation protocol of protocorm-like body was developed by employing orthogonal experimental design method.Compact callus were incuced in the axillary buds of the stem node explants on MS basal medium supplemented with 2.0mg/L 6-BA,0.4mg/L NAA and 0.6mg/L KT.Maximum rate of induction and multiplication（4.25 shoots per explant） were observed when PLB were sub-cultured on MS medium supplemented with 4.0mg/L 6-BA,1.0mg/L KT ...
