期刊论文

中文

植物遗传资源学报

1672-1810

2017

18

1

117-124

10.13430/j.cnki.jpgr.2017.01.015

国家转基因生物新品种培育重大科技专项（2016ZX08002-004）；

本校为第一机构

植物原生质体广泛应用于植物基因功能研究中,包括瞬时基因表达、亚细胞定位、蛋白互作和蛋白活性分析等。当前,小麦基因的亚细胞定位和功能分析,大多利用模式植物拟南芥等异源的原生质体,易于造成研究结果的不准确。为避免这种情况,小麦原生质体制备及高效转化体系的建立与应用是必需的。在PEG介导的小麦原生质体转化过程中,原生质体分泌的核酸酶大量降解质粒DNA,转化效率的提高因此受到阻碍。为了建立小麦原生质体的高效转化体系,本文测试了抑制胞外核酸酶活性的因素和提高质粒DNA浓度等多个条件对转化效率的影响。结果表明,转化过程中加入双倍用量的质粒DNA进行转化,且始终保持低温环境(1 ℃)...

A plant protoplast system can be used to study transient gene expression,protein subcellular localization, protein-protein interaction, protein activity and other functional analysis. At present, heterologous protoplast systems such as Arabidopsis have been mostly applied to study wheat gene subcellular localization and others function and may show aberrant traits. To avoid this situation,it is necessary to set up and apply the homologous proto- plast systems. In wheat, the PEG-mediated plasmid DNA protoplast transformation is hampered by the release of nucleases from the protoplasts,which lea...