期刊论文

Establishment and Application of SSR Molecular Marker Identification System for Common Bean(Phaseolus vulgaris L.)

中文

植物遗传资源学报

1672-1810

2019

20

6

1494-1505

10.13430/j.cnki.jpgr.20190702001

农业行业标准制订和修订 国家自然科学基金(31772325) 湖南省自然科学基金(2017JJ2118)。

本校为第一机构

为了保护育种家合法权益、规范种业市场、提高种子市场监管效率等,本研究拟建立一套普通菜豆品种SSR分子标记鉴定体系。首先,遴选12个产地不同、植物学形态差异较大的普通菜豆品种,通过聚丙烯酰胺凝胶电泳筛选出120对多态性高、条带清晰的SSR引物;然后,利用荧光毛细管电泳技术对筛选出的引物多态性在96个普通菜豆品种中进一步检测,最终获得在菜豆基因组上均匀分布、多态性高、峰型稳定的33对SSR核心引物。在此基础上,依据扩增片段长度和荧光标记颜色对核心引物进行分组,并设置参照品种以校正不同实验批次和平台之间的误差,建立了基于SSR标记的品种鉴定体系。同时,构建了包含申请品种、保护品种、...

In recent years,the new varieties breeding of common bean has been developing rapidly,and the number of new varieties and applications for Plant Variety Protection(PVP)has been increasing.In order to protect the legitimate rights and interests of breeders,regulate the seed market and improve the efficiency of the seed market supervision,this study intends to establish common bean identification system based on a set of SSR molecular markers.Firstly,12 common bean varieties with different origins and botanical morphology were selected,and 120 SSR markers with high polymorphism and clear bands w...