期刊论文

中文

植物遗传资源学报

1672-1810

2022

23

2

605-613

10.13430/j.cnki.jpgr.20211013001

31972256:国家自然科学基金 2016YFD0101103-2:国家重点研发计划

本校为第一机构

以籼稻品系75-1-127作为广谱持久抗稻瘟病基因Pi9的供体亲本，先后以籼型三系保持系三香B及其不育系三香A为受体亲本，利用Pi9基因内共显性In Del标记Co In DF1R1开展分子标记辅助选择连续回交育种，定向改良三香B和三香A的稻瘟病抗性。以三香B、三香A、75-1-127和感病对照CO39为材料，用来自国内外不同稻区的28份稻瘟菌菌株进行室内苗瘟接种试验，结果表明，75-1-127的抗性频率为85.71%，三香A和三香B的抗性频率均为28.57%。共显性标记Co In DF1R1从75-1-127基因组中扩增出一条320 bp的清晰条带，而从三香A和三香B基因组中的扩增产物约410 bp，多态性明显且稳定。用Co In DF1R1开展了连续10年的...

To improve blast resistance of the rice cytoplasmic male sterile(CMS)line Sanxiang A and its maintainer Sanxiang B,a PCR-based co-dominant In Del marker Co In DF1 R1 was developed to select the broadspectrum and durable blast resistance gene Pi9 in breeding practice. By performing the greenhouse inoculation assay using twenty-eight Magnaporthe oryzae strains isolated from different rice areas in China and abroad,the Pi9 gene donor rice line 75-1-127 showed much higher resistance frequency(85.71%)than that of two acceptor parents Sanxiang A and Sanxiang B(28.57%). The Pi9 functional marker Co I...