期刊论文

Establishment and Application of Mustard Variety Identification System Based on SSR Markers(Brassica juncea L.)

中文

植物遗传资源学报

1672-1810

2021

22

03

758-770

10.13430/j.cnki.jpgr.20201014002

湖南省发改委科技专项课题（湘发改投资[2014]658号）～～；

本校为第一机构

利用SSR分子标记建立芥菜品种鉴定体系,旨在为我国芥菜育成品种鉴定提供高通量、快速鉴定方法。选取芥菜16个变种代表性品种用于SSR引物初筛,通过聚丙烯酰胺凝胶电泳从432对引物中筛选出84对条带清晰、稳定性好、多态性高的芥菜SSR引物,并对筛选出的引物5′端进行荧光标记。另外选取96份品种对上述84对引物进行复筛,利用基因分析仪对所有扩增片段大小进行分析,依据各引物的扩增稳定性、峰型易读取程度、多态性及染色体位置等,最终筛选出25对SSR引物作为核心引物,并为其设置参照品种以消除不同试验批次或平台等因素引起的误差。根据荧光颜色和扩增片段大小将25对核心引物分成6组,建立基于SSR标记...

This study attempted to establish a high-throughput and rapid method using SSR markers,in order to clarify the mustard varieties(Brassica juncea L.)released from China.Sixteen representative mustard varieties were used to screen SSR primers showing polymorphism.Out of 432 pairs of tested primers,84 were detected with higher polymorphism and good repeatability,and they were subjected for synthesizing the fluorescentlabeled primers.We further genotyped 96 varieties with updated versions of primers using gene analyzer.Based on the amplification stability,the readability of peaks,polymorphism and ...