期刊论文

Zhou, Jian-da

英文

中国有色金属学报(英文版)

1003-6326

2013

23

7

2079-2084

10.1016/S1003-6326(13)62699-0

Project（2011JQ028）supported by the Fundamental Research Funds for the Central Universities,China Projects（2008SK3114,2010SK3113）supported by Hunan Provincial Science＆Technology Plan,China Project（B2007086）supported by Science＆Research Funds of Hunan Health Department,China Project（12JJ5057）supported by Natural Science Foundation of Hunan Province,China Projects（XCX1119,XCX12073）supported by University Students Innovative Experiment Plan Project of Hunan Agricultural University,China

本校为第一机构

Highly biocompatible superparamagnetic Fe3O4 nanoparticles were synthesized by amide of folic acid (FA) ligands and the NH2-group onto the surface of Fe3O4 nanoparticles. The as-synthesized folate-conjugated Fe3O4 nanoparticles were characterized by X-ray diffraction diffractometer, transmission electron microscope, FT-IR spectrometer, vibrating sample magnetometer, and dynamic light scattering instrument. The in vivo labeling effect of folate-conjugated Fe3O4 nanoparticles on the hepatoma cells was investigated in tumor-bearing rat. The results demonstrate that the as-prepared nanoparticles h...

采用酰胺化反应方法,通过Fe3O4纳米颗粒表面氨基基团和与肿瘤细胞具有高度亲和力的叶酸分子的羧基间反应进行偶联得到目标材料,利用X射线衍射、傅立叶红外光谱仪、透射电镜、振动样品磁强计、动态光散射仪等对所合成的材料进行表征,且以瘤鼠为动物模型,考察其活体肝癌细胞标记效果和MRI成像功能。结果表明,合成的材料为立方相的Fe3O4,粒径约8nm,水合直径约25.7nm,呈近似球形形貌,表面分布有羧基等功能基团,呈超顺磁特性,饱和磁化强度为51A·m2/kg,能靶向进入肝癌细胞内,提高肿瘤与周围正常组织的MRI成像信号对比。实验证实,叶酸偶联的超顺磁Fe3O4纳米颗粒,不仅细胞毒性小,而且因其表面的叶酸与叶...