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Effect of grape seed extract on the oxidative and proliferative status of porcine intestinal epithelial cells [Üzüm cekirdeği ekstraktının domuz barsak epitel hücreleri üzerine oksidatif ve proliferatif etkileri]

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成果类型:
期刊论文
作者:
Kang, Jinhe;Yang, Tan;Wu, Zhaoliang;Yang, Jing;Xiong, Min;...
通讯作者:
Tan, Zhiliang;Sun, Zhihong
作者机构:
[Yang, Tan; Tan, Zhiliang; Kang, Jinhe] Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, South Cent Expt Stn Anim Nutr & Feed Sci, Minist Agr,Inst Subtrop Agr,Hunan Res Ctr Livesto, Changsha 410125, Hunan, Peoples R China.
[Yang, Tan; Wu, Zhaoliang; Xiong, Min; Yang, Jing; Sun, Zhihong] Southwest Univ, Key Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.
[Xiao, Wenjun] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Tan, Zhiliang] C
[Sun, Zhihong] S
Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, South Cent Expt Stn Anim Nutr & Feed Sci, Minist Agr,Inst Subtrop Agr,Hunan Res Ctr Livesto, Changsha 410125, Hunan, Peoples R China.
Southwest Univ, Key Lab Biofeed & Mol Nutr, Chongqing 400715, Peoples R China.
语种:
英文
关键词:
caspase 3;caspase 8;cholecystokinin octapeptide;copper zinc superoxide dismutase;glutathione;glutathione peroxidase 1;glutathione transferase;grape seed extract;manganese superoxide dismutase;messenger RNA;peroxiredoxin 5;protein Bax;protein bcl 2;protein p53;thioredoxin;thioredoxin reductase;tumor necrosis factor alpha receptor;analysis of variance;animal cell;animal experiment;animal model;antioxidant assay;antiproliferative activity;apoptosis;Article;B cell lymphoma;cancer cell;cell proliferation;cell survival;comparative study;controlled study;drug efficacy;gene expression;intestine epithelium cell;nonhuman;observational study;oxidation;oxidative stress;porcine model;protein expression;real time polymerase chain reaction;receptor down regulation
期刊:
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
ISSN:
1300-6045
年:
2018
卷:
24
期:
4
页码:
511-517
基金类别:
The present study was supported by the National Natural Science Foundation of China (grants 31320103917, 30600436 and 31201826), “Strategic Priority Research Program-Climate Change: Carbon Budget and Relevant Issues” of the Chinese Academy of Sciences (Grant XDA05020700), the National Program on Key Basic Research Project of China (2013CB127303 and 2013CB127304).
机构署名:
本校为其他机构
院系归属:
园艺园林学院
摘要:
The aim of this study was to investigate the oxidative and proliferative effects of grape seed extract (GSE). Piglet intestinal epithelial cells (IPEC1) were selected as an unstressed cell model, or they were exposed to 400 μM H2O2 to establish a H2O2-stimulated cell model. The glutathione (GSH) and total antioxidant capacity in response to GSE addition were tested in the unstressed and H2O2-stimulated cell models. The relative mRNA levels of antioxidant or antioxidant enzymes and apoptosis-related genes were measured by Real-Time RT-PCR. In t...

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