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A conserved residue in the P2X4 receptor has a nonconserved function in ATP recognition

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成果类型:
期刊论文
作者:
Chen, Ping-Fang;Ma, Xue-Fei;Sun, Liang-Fei;Tian, Yun;Fan, Ying-Zhe;...
通讯作者:
Li, Changzhu;Yu, Ye;Wang, Jin
作者机构:
[Sun, Liang-Fei; Yu, Ye; Chen, Ping-Fang] Shanghai Jiao Tong Univ, Inst Med Sci, Dept Pharmacol & Chem Biol, Sch Med, Shanghai, Peoples R China.
[Guo, Chang-Run; Yu, Ye; Wang, Jin; Ma, Xue-Fei] China Pharmaceut Univ, Sch Basic Med & Clin Pharm, Nanjing, Peoples R China.
[Tian, Yun; Ma, Xue-Fei] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha, Peoples R China.
[Fan, Ying-Zhe] Shanghai Univ Chinese Tradit Med, Putuo Hosp, Shanghai, Peoples R China.
[Li, Changzhu; Xiao, Zhihong; Li, Peiwang] Hunan Acad Forestry, State Key Lab Utilizat Woody Oil Resource, Changsha, Peoples R China.
通讯机构:
[Changzhu Li; Jin Wang] S
[Ye Yu] D
Department of Pharmacology and Chemical Biology, Institute of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, China<&wdkj&>School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, China<&wdkj&>State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, China<&wdkj&>School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, China<&wdkj&>State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha, China
语种:
英文
关键词:
channel gating;electrophysiology;ion channel;molecular dynamics;purinergic receptor;side chain orientation;structure-function
期刊:
Journal of Biological Chemistry
ISSN:
0021-9258
年:
2021
卷:
296
页码:
100655
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [32000869, 31570832, 31971146, 81603409, 31971042]; Natural Science Foundation of Jiangsu ProvinceNatural Science Foundation of Jiangsu Province [BK20202002]; Innovation and Entrepreneurship Talent Program of Jiangsu Province; Open Project of State Key Laboratory of Natural Medicines [SKLNMZZRC201801]; Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [2018JJ1012]; Guangxi Funds for Distinguished Experts; State Key Laboratory of Utilization of Woody Oil Resource [2019XK2002]
机构署名:
本校为其他机构
院系归属:
生物科学技术学院
摘要:
Highly conserved amino acids are generally anticipated to have similar functions across a protein superfamily, including that of the P2X ion channels, which are gated by extracellular ATP. However, whether and how these functions are conserved becomes less clear when neighboring amino acids are not conserved. Here, we investigate one such case, focused on the highly conserved residue from P2X4, E118 (rat P2X4 numbering, rP2X4), a P2X subtype associated with human neuropathic pain. When we compared the crystal structures of P2X4 with those of other P2X subtypes, including P2X3, P2X7 and AmP2X, ...

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