期刊论文

Effects of Euphorbiae humifusae total flavonoids on immunity and mRNA expression of some cytokines in mice

中文

中兽医医药杂志

1000-6354

2010

29

5

22-26

10.3969/j.issn.1000-6354.2010.05.006

湖南省科学技术厅项目（06FJ3107）；

本校为第一机构

探讨地锦草总黄酮对小鼠免疫功能及细胞因子IL-2、IL-12、IFN-γ、TNF-α mRNA表达影响.将小鼠随机分成4组:地锦草总黄酮高剂量组(H组)、中剂量组(M组)、低剂量组(L组)和纯水对照组(C组),连续灌胃9 d后,检测其单核巨噬细胞吞噬功能、2%绵羊红细胞诱导的小鼠迟发型变态反应、肝脏指数和脾脏指数,并用逆转录酶-聚合酶链式反应的方法检测脾脏IL-2、IL-12、IFN-γ和TNF-α mRNA表达水平.结果显示,地锦草总黄酮3个剂量组脾脏指数、廓清指数(K)、吞噬指数(α)、24 h足跖增厚值均显著高于C组;M组和H组肝指数显著高于C组(P

Objective： To investigate the effect of Euphorbiae humifusa total flavonoids on immunity and mRNA expression of some cytokines,such as IL-2,IL-12,IFN-γ and TNF-α in mice.Methods： Kunming mice were divided into 4 groups randomly： the mice in control group were treated with water（c）,other 3 groups were treated with high（H）,moderate（M）and low（L）dose of Euphorbiae humifusa total flavonoids for 9 d,respectively.Then phagocytosis of mononuclear macrophage,DTH induced by sheep red blood cell,liver index and spleen index were determined.Th...