期刊论文

Study on the immunocompetence of polysaccharides from Hedyotis diffusa Willd. in vitro

中文

中兽医医药杂志

1000-6354

2015

34

3

17-20

10.13823/j.cnki.jtcvm.2015.03.004

2011DFA30490:科技部国际科技合作专项 2012FJ1004:湖南省科技重大专项

本校为第一机构

目的:探讨白花蛇舌草多糖对小鼠胸腺和脾脏体外免疫功能的影响。方法:采用MTT法研究不同浓度（500μg/m L、250μg/m L、200μg/m L、150μg/m L、100μg/m L、50μg/m L、25μg/m L）的白花蛇舌草多糖对体外培养的小鼠胸腺和脾脏淋巴细胞的代谢及其与Con A或LPS协同诱导的转化作用、小鼠胸腺和脾脏巨噬细胞吞噬活性及自然杀伤细胞活性的影响。结果:白花蛇舌草多糖可显著提高脾脏巨噬细胞吞噬功能（P

Objective: To investigate the effect of polysassharides from Hedyotis diffusa Willd.(PHDW) on immune function of mouse thymocytes and splenocytes in vitro. Methods: MTT assay was used to study the effect of different concentration of PHDW on mouse thymus and spleen lymphocyte metabolism, Con A synergy with LPS-induced transformation, and macrophage phagocytosis and NK cell activity of mouse thymocytes and splenocytes. Results: PHDW could significantly improve splenic macrophage metabolism and phagocytosis(P