期刊论文

Optimization on cellulase-assisted extraction of total flavonoids from Fructus Rosae Laevigatae by single factor experiment

中文

中兽医医药杂志

1000-6354

2016

35

1

29-32

10.13823/j.cnki.jtcvm.2016.01.008

湖南农业大学动物医学院大学生创新性实验计划项目；

本校为第一机构

为优化纤维素酶辅助提取金樱子总黄酮的提取工艺,通过单因素试验考察乙醇浓度、液料比、提取时间、纤维素酶用量、p H值、提取次数等6个因素对金樱子总黄酮得率的影响,结果其最佳提取工艺条件为：乙醇浓度40%、液料比30∶1、提取时间120 min、纤维素酶用量1.0%、p H值6、提取温度60℃、提取2次,在该提取条件下,金樱子总黄酮得率为（79.30±1.59）mg/g。提示纤维素酶辅助提取金樱子总黄酮可行、得率较高。

To optimize the process for cellulase-assisted extraction of total flavonoids from Fructus Rosae Laevigatae, the effects of ethanol concentration, ratio of liquid to material, extraction time, cellulase dosage, pH value and extraction times on the yield of total flavonoids from Fructus Rosae Laevigatae was investigated by single factor test. The results showed that the optimum extraction conditions were 40%of ethanol, ratio of liquid to material of 30∶1, extracting 2 times and 120 min every time, cellulase dosage of 1.0%, pH of 6, and extracti...