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茶树甲基化EGCG生物合成相关OMTs基因的鉴定及表达

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成果类型:
期刊论文
作者:
罗勇;温贝贝;陈燕;曹桂萍;雷淑敏;...
通讯作者:
Wang, K.
作者机构:
[曹桂萍; 雷淑敏; 陈燕; 罗勇] School of Chemistry and Environmental Science, Xiangnan University, Chenzhou, 423000, China
Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, 410128, China
National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Changsha, 410128, China
[李群] School of Pharmacy, Xiangnan University, Chenzhou, 423000, China
[温贝贝; 王坤波] Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, 410128, China, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Changsha, 410128, China
通讯机构:
[Wang, K.] K
Key Laboratory of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, China
语种:
中文
关键词:
茶树;甲基化EGCG;OMT基因;转录组;定量表达
关键词(英文):
Camellia sinensis;O-methyltransferase EGCG;OMT gene;Real-time quantitative PCR;Transcriptome
期刊:
应用与环境生物学报
ISSN:
1006-687X
年:
2022
卷:
28
期:
1
页码:
26-33
基金类别:
收稿日期 Received: 2021-07-13 接受日期 Accepted: 2021-10-08 湖南省自然科学基金(2020JJ5525)和湖南省教育厅科学研究项目(19C1720)资助 Supported by the Natural Science Foundation of Hunan Province (2020JJ5525) and the Research Foundation of Education Bureau of Hunan Province(19C1720) 通信作者 Corresponding author (E-mail: wkboo163@163.com)
机构署名:
本校为通讯机构
院系归属:
园艺园林学院
摘要:
茶树O-甲基转移酶(OMT)可催化甲基化EGCG的生物合成.为进一步获得甲基化EGCG生物合成的关键OMT基因,以不同甲基化EGCG含量的茶树品种金观音(JGY)、金牡丹(JMD)和福鼎大白(FD)为试验材料,基于茶树基因组和转录组数据,通过生物信息学方法,对筛选获得的OMTs基因进行染色体定位、基因结构特征、编码蛋白特性和系统进化关系分析,同时采用实时荧光定量PCR验证茶树OMTs在不同茶树品种的相对表达水平.结果显示,共鉴定出5个在金观音与福鼎大白以及金牡丹与福鼎大白比较组合中显著上调表达的OMT基因,其编码序列(CDS)全长和编码氨基酸的数目分别在528-1 122 bp和175-373 aa之间,命名为Cs...
摘要(英文):
O-methyltransferase (OMT) catalyzes the biosynthesis of O-methylated EGCG in Camellia sinensis. To further obtain the key OMT genes for O-methylated EGCG biosynthesis, three tea cultivars including “Fudingdabai (FD)”, “Jinmudan (JMD)”, and “Jinguanyin (JGY)” with different O-methylated EGCG contents, were used as the experimental materials in this study. Based on the genomic and transcriptomic data of tea plants, the OMT genes were analyzed using bioinformatics methods for gene identification, chromosomal localization, gene structural cha...

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