期刊论文

Isolation and identification of Bacillus tequilensis JN-369 and antimicrobial substance analysis

中文

农药学学报

1008-7303

2019

21

1

52-58

10.16801/j.issn.1008-7303.2019.0007

国家重点研发计划项目(2016YFD0300700) 中央财政专项(2014ZX0800102B) 公益性行业(农业)科研专项(201203014) 湖南省“十二五”重点学科(0904).

本校为第一机构

为筛选可用于防治由稻瘟病菌所致水稻稻瘟病的生防菌株资源,采用稀释涂布平板法,从感病水稻品种湘早籼24号的健康植株茎叶中分离获得了1株拮抗细菌JN-369,结合形态学观察、生理生化鉴定及16S rDNA序列分析对其菌种进行了鉴定,采用平板对峙法研究了JN-369菌株的抑菌谱,并初步测定了JN-369中挥发性有机物(VOCs)、蛋白类粗提物及脂肽类粗提物对稻瘟病菌的抑制作用。结果表明:JN-369菌株为特基拉芽孢杆菌Bacillus tequilensis,其对稻瘟病菌具有显著抑制作用,对菌丝生长的抑制率达80.46% ± 0.83%;同时该菌株对供试的辣椒胶孢炭疽菌、烟草赤星病菌及黄瓜疫病菌等植物病原真菌和卵菌均有抑制作用;但对...

In order to screen the resources of biocontrol bacteria strains for the control of rice blast caused by Magnaporthe oryzae, a bacteria JN-369 was isolated from the healthy rice plants of the susceptible rice cultivar Xiangzaoxian 24 by dilution plate method. And its activity against mycelial growth of M. oryzae was tested by the plate confrontation method. The strain JN-369's taxonomic status was identified based on morphological characteristics, physiological, biochemical characteristics and 16S rDNA sequence analysis. The antifungal spectrum ...