期刊论文

中文

水产科技情报

1001-1994

2022

49

3

137-142

10.16446/j.fsti.20210300209

湖南省科技重大专项课题（2017NK1031）； 湖南农业大学双一流建设项目（SYL201802020）；

本校为第一机构

为了解洞庭湖区养殖克氏原螯虾的遗传多样性，从线粒体Cytb和COⅠ基因入手，经基因组DNA提取、PCR扩增、序列测定和拼接，比较分析了岳阳华容县和益阳南县2处主养殖区内共11个采集点的克氏原螯虾遗传结构。试验结果显示，在华容县的6个采集点中，基于Cytb基因的克氏原螯虾的单倍型多样性指数为0.506,核苷酸多样性指数为0.272 65;基于COⅠ基因的克氏原螯虾的单倍型多样性指数为0.258,核苷酸多样性指数为0.136 1。在南县的5个采集点中，基于Cytb基因的克氏原螯虾的单倍型多样性指数为0.467,核苷酸多样性指数为0.259 74;基于COⅠ基因的克氏原螯虾的单倍型多样性指数为0.244,核苷酸多样性指数为0.12...

In order to understand the genetic diversity of Procambarus clarkii cultured in Dongting Lake area,the genetic structures of P.clarkii collected from 11 collection points in Huarong County of Yueyang and Nanxian County of Yiyang were compared and analyzed by extracting genomic DNA,PCR amplification,sequencing and splicing according to Cytb and COⅠgenes in mitochondria.Among the six collection points in Huarong,the haplotype diversity index and nucleotide diversity index of P.clarkii based on Cytb gene were 0.506 and 0.27265.The haplotype diver...