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Functional Identification of HhUGT74AG11—A Key Glycosyltransferase Involved in Biosynthesis of Oleanane-Type Saponins in Hedera helix

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成果类型:
期刊论文
作者:
Yu, Han;Zhou, Jun;Zhang, Jing;He, Xinyi;Peng, Siqing;...
通讯作者:
Tang, Q;He, YD
作者机构:
[Zhou, Jun; Peng, Siqing; Tang, Qi; Yu, Han; He, Xinyi; Zhang, Jing; Zhong, Xiaohong; Ling, Hao; Dong, Zhuang; Tang, Q] Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China.
[Tian, Yun; Lu, Xiangyang; He, YD; He, Yuedong; Guan, Guiping] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
通讯机构:
[He, YD ; Tang, Q ] H
Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China.
Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Peoples R China.
语种:
英文
关键词:
UDP-glycosyltransferase;substrate hybridity;biosynthesis;oleanane-type saponin;Hedera helix
期刊:
International Journal of Molecular Sciences
ISSN:
1661-6596
年:
2024
卷:
25
期:
7
页码:
4067-
基金类别:
H.Y.: Formal analysis, Methodology, Investigation, writing—original draft preparation, Validation, Visualization. Q.T.: Conceptualization, Validation—review and editing. J.Z. (Jun Zhou): Investigation, Writing—review and editing. J.Z. (Jing Zhang): funding acquisition, Writing—review and editing. X.H.: Investigation, Validation. S.P.: Formal analysis, Validation. H.L.: Formal analysis. Z.D.: Visualization. X.L.: Investigation. Y.T.: Formal analysis. G.G.: Formal analysis. Y.H.: Conceptualization, Validation, Writing—review and editing, Visualization, Supervision, Funding acquisition, Resources. X.Z.: Conceptualization, Validation, Formal analysis, Funding acquisition, Investigation. All authors have read and agreed to the published version of the manuscript. This research was funded by the China Postdoctoral Science Foundation (2023M741143), Hunan Province Natural Science Foundation (2021JJ30320), the Scientific Research Fund of Hunan Provincial Education Department (23B0228), the Research Innovation Project of Postgraduates in Hunan Agricultural University (CX20230706), and the Seed Industry Innovation Project of Hunan province of China (2021NK1005). Postgraduate Scientific Research Innovation Project of Hunan Province (QL 20220172).
机构署名:
本校为通讯机构
院系归属:
园艺园林学院
生物科学技术学院
摘要:
Hedera helix is a traditional medicinal plant. Its primary active ingredients are oleanane-type saponins, which have extensive pharmacological effects such as gastric mucosal protection, autophagy regulation actions, and antiviral properties. However, the glycosylation-modifying enzymes responsible for catalyzing oleanane-type saponin biosynthesis remain unidentified. Through transcriptome, cluster analysis, and PSPG structural domain, this study preliminarily screened four candidate UDP-glycosyltransferases (UGTs), including Unigene26859, Unigene31717, CL11391.Contig2, and CL144.Contig9. In i...

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