Homologous recombination technology generated recombinant pseudorabies virus expressing EGFP facilitates to evaluate its susceptibility to different cells and screen antiviral compounds

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Tan, Lei;Shu, Xianghua;Xu, Kaiwen;Liao, Fan;Song, Chunlian;...

通讯作者：

Yao, J.

作者机构：

[Wang, Aibing; Xu, Kaiwen; Liao, Fan; Tan, Lei; Duan, Deyong] Hunan Agr Univ HUNAU, Coll Vet Med, Hunan Prov Key Lab Prot Engn Anim Vaccines, Changsha 410128, Peoples R China.

[Shu, Xianghua; Song, Chunlian] Yunnan Agr Univ, Coll Anim Med, Kunming 650201, Yunnan, Peoples R China.

[Yao, Jun; Yang, Shibiao] Yunnan Anim Sci & Vet Inst, Yunnan Trop & Subtrop Anim Virus Dis Lab, Kunming 650224, Yunnan, Peoples R China.

[Wang, Aibing] PCB Biotechnol LLC, Rockville, MD 20852 USA.

通讯机构：

Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, China

语种：

英文

关键词：

Pseudorabies virus;EGFP;Homologous recombination;Infectivity;Antiviral agents

期刊：

Research in Veterinary Science

ISSN：

0034-5288

年：

2022

卷：

145

页码：

125-134

DOI：

10.1016/j.rvsc.2022.02.005

基金类别：

Hunan Provincial Natural Science Foundation of China [2020JJ4041]; Postgraduate Scientific Research Innovation Project of Hunan Province [CX20200659]; National Key Research and Development Program of China [2017YFD0501800]; Major Specialized Projects of Yunnan Science and Technology [202102AE090007]

机构署名：

本校为第一机构

院系归属：

动物医学院

摘要：

Pseudorabies virus (PRV) is considered as an emerging zoonotic pathogen since its isolation from a human case. Meanwhile, the disease caused by PRV infection has led huge economic losses to Chinese pig industry since 2011. In this study, we constructed a recombinant PRV stably expressing the enhanced green fluorescent protein (EGFP) by homologous recombination technology for evaluating its susceptibility to different human cell lines and screening antiviral compounds. Stably expressed EGFP by this designed rPRVHuN-EGFP virus was confirmed in the infected cells, moreover, the growth kinetics of...

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Homologous recombination technology generated recombinant pseudorabies virus expressing EGFP facilitates to evaluate its susceptibility to different cells and screen antiviral compounds

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