A rapid, label-free, and multifunctional fluorescent probe for simultaneous detection of multiple targets was fabricated based on G-quadruplexes and DNA-templated silver nanoclusters (AgNCs). In this work, a probe with two capitated recognized regions was coupled with a locked G-quadruplex at the 5′-terminus and dark AgNC at the 3′-terminus. Upon the addition of the virus subtype H5N1 gene or microRNA-141, only the sequence of the G-quadruplex was released and bound with Thioflavin T (ThT) for a specific fluorescent response. On the contrary,...
