期刊论文

Moriguchi, Takaya

英文

Biochemical and Biophysical Research Communications

0006-291X

2008

366

1

162-167

10.1016/j.bbrc.2007.11.099

This work was supported in part by Grants-in-Aid from the Japan Society for the Promotion of Science (JSPS). We thank Drs I. Suzuki, Y. Shiraiwa, H. Ezura, and T. Mizoguchi (University of Tsukuba, Japan) and Dr. K. Sugimoto (National Institute of Agrobiological Sciences) for their technical suggestions. We thank Dr. S. Terakami (University of Tsukuba, Japan) for his technical assistance with the computational analysis.

本校为其他机构

Several lines of evidence suggest different allocations of the physiological roles of aminopropyl transferase genes, SPMS and ACL5 in plants. To get deeper insights into the physiological role of apple ACL5 (MdACL5), we performed yeast two-hybrid (Y2H) assay to identify proteins which interact with MdACL5. After intense screening processes, including the swapping of the bait and prey vectors and in vitro coimmunoprecipitation, we identified three MdACL5-interacting proteins: putative translation elongation factor 1A (eEF-1A), putative S-adenosy...