期刊论文

Wang Yan-Ling

中文

生物化学与生物物理进展

1000-3282

2006

33

5

492-499

10.3321/j.issn:1000-3282.2006.05.013

30370542:中国科学院项目（非规范项目） 30530760:中国科学院项目（非规范项目）

本校为其他机构

E-钙粘素是在胚胎发育中最早表达的分子之一，它可以与Catenin家族成员形成钙粘素／Catenin复合物参与多种细胞功能，对于胚胎植入和胎盘发生具有重要作用．通过RT-PCR、免疫组织化学、细胞粘附分析等方法，在人正常妊娠和输卵管妊娠母胎界面上，发现E-钙粘素主要定位于绒毛细胞滋养层细胞和滋养层细胞柱，从滋养层细胞柱近端向远端，其蛋白质水平逐渐降低．正常胎盘组织中E-钙粘素水平在妊娠早期较高，妊娠中期直至分娩期均维持低水平．在体外培养的人正常胎盘细胞滋养层细胞系（NPC细胞）中，转化生长因子β（TGFβ1）显著上调E-钙粘素蛋白和mRNA的表达，并呈现时间和剂量依赖性，同时，TGFβ...

E-cadherin was one of the early expressed genes during embryo development. The intact form of E-cadherin can link to cytoskeleton via β catenin, and the complex plays essential roles in cell adhesion. Evidences indicated the essential functions of E-cadherin in processes of embryoic implantation and placentation. Immunohistochemistry and RT-PCR were used to identify the localization of E-cadherin in human placenta. It was found that E-cadherin was expressed mainly in villous cytotrophoblasts and trophoblast column, with the immunoreactivity de...