期刊论文

Hu, Xinxi

英文

Molecular and Cellular Probes

0890-8508

2019

47

101438

10.1016/j.mcp.2019.101438

This research was supported by the China Agricultural Research System Program (CARS-09-ES16) and the Non-Profit Industry Financial Program (20130328), Ministry of Agriculture of the People's Republic of China. This research was supported by the China Agricultural Research System Program ( CARS-09-ES16 ) and the Non-Profit Industry Financial Program ( 20130328 ), Ministry of Agriculture of the People's Republic of China .

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In this study, a new SYBR Green qPCR (qRT-PCR) and a nested RT-PCR (nRT-PCR) were developed to detect Potato mop-top virus (PMTV) in potato tuber tissues. The SYBR Green qRT-PCR and nRT-PCR assays were approximately 104- and 103- fold more sensitive than the conventional RT-PCR assay. The progeny tubers derived from PMTV-infected potato tubers were tested by conventional RT-PCR, SYBR Green qRT-PCR and nRT-PCR assays. Of the 17 samples, 9 (52.9%) were positive for PMTV by conventional RT-PCR, 11 (64.7%) were positive by nRT-PCR, and 17 (100%) we...