期刊论文

Hu, Xinxi

英文

Molecular and Cellular Probes

0890-8508

2019

47

101438

10.1016/j.mcp.2019.101438

China Agricultural Research System Program [CARS-09-ES16]; Non-Profit Industry Financial Program, Ministry of Agriculture of the People's Republic of China [20130328]

本校为第一且通讯机构

In this study, a new SYBR Green qPCR (qRT-PCR) and a nested RT-PCR (nRT-PCR) were developed to detect Potato mop-top virus (PMTV) in potato tuber tissues. The SYBR Green qRT-PCR and nRT-PCR assays were approximately 10(4)- and 10(3)-fold more sensitive than the conventional RT-PCR assay. The progeny tubers derived from PMTV-infected potato tubers were tested by conventional RT-PCR, SYBR Green qRT-PCR and nRT-PCR assays. Of the 17 samples, 9 (52.9%) were positive for PMTV by conventional RT-PCR, 11 (64.7%) were positive by nRT-PCR, and 17 (100%) were positive by SYBR Green qRT-PCR. Compared to ...