Optimized conditions for preserving stability and integrity of porcine circovirus type2 virus-like particles during long-term storage

首页 > 成果 > 详情

认领

导出

Link by DOI

反馈

作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Wang, Naidong;Zhang, Yan;Lei, Xinnuo;Yu, Wanting;Zhan, Yang;...

通讯作者：

Yang, Yi;Jiang, Ping

作者机构：

[Wang, Naidong; Zhan, Yang; Wang, Dongliang; Wang, Aibing; Yu, Wanting; Zhang, Jiaxin; Zhang, Yan; Lei, Xinnuo; Yang, Yi] Hunan Agr Univ, LFP, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.

[Wang, Naidong; Zhan, Yang; Wang, Dongliang; Wang, Aibing; Yu, Wanting; Zhang, Jiaxin; Zhang, Yan; Lei, Xinnuo; Yang, Yi] Hunan Agr Univ, RCRV, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.

[Xiao, Lehui] Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China.

[Jiang, Ping] Nanjing Agr Univ, Key Lab Anim Dis Diagnost & Immunol, Minist Agr, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China.

通讯机构：

[Yang, Yi] H

[Jiang, Ping] N

Hunan Agr Univ, LFP, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.

Hunan Agr Univ, RCRV, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.

Nanjing Agr Univ, Key Lab Anim Dis Diagnost & Immunol, Minist Agr, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China.

语种：

英文

关键词：

Porcine circovirus type 2 (PCV2);Virus-like particles (VLPs);Osmolytes;Stability

期刊：

Journal of Virological Methods

ISSN：

0166-0934

年：

2017

卷：

243

页码：

146-150

DOI：

10.1016/j.jviromet.2017.01.021

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270819, 31571432]; Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [13JJ1022/S2013J5050/2015JC3097]; Research Foundation of Hunan Provincial Education Department, China [15A086]; Planned Science and Technology Project of Hunan Province [2014FJ2011]; Postgraduate Research and Innovation Project of Hunan Provinc [CX2016B285, CX2016B314]; Ministry of Agriculture for swine diseases controlling techniques [CARS-36]

机构署名：

本校为第一且通讯机构

院系归属：

动物医学院

摘要：

Although porcine circovirus type 2 (PCV2) virus-like particles (VLPs) have been successfully harvested from various protein expression systems, conditions to promote their stability and integrity during long-term storage have not been well defined since only the intact VLPs, instead of the monomeric capsid protein (Cap), can induce neutralizing antibodies in pigs in previous studies. In this study, freshly prepared PCV2 VLPs were stored in several media (various concentrations of NaCl, sorbitol, sucrose and trehalose) at three temperatures (4 °...

反馈

产权有误：本人成果被他人认领

数据有误：数据基本信息有误

归属有误：成果的院系归属、机构署名归属有误

其他原因：

验证码：

看不清楚，换一个

确定

取消

成果认领

标题：

用户	作者	通讯作者	--
	请选择	请选择	--

确定

取消

Optimized conditions for preserving stability and integrity of porcine circovirus type2 virus-like particles during long-term storage

反馈

成果认领

提示

该栏目需要登录且有访问权限才可以访问