期刊论文

Shen, GL

英文

Analytical and Bioanalytical Chemistry

1618-2642

2003

376

6

902-907

10.1007/s00216-003-1989-8

Acknowledgements This work was supported by the National Natural Science Foundation of China (Grants Nos. 29975006, 20075006, 70171055, 50179011), the Foundation of The National 863 High Technologies Research of China (No 2001AA644020), the Foundation for Ph.D Thesis Research (No. 20010532008) and the Foundation of Hunan province (Grants Nos. 01JJY1003, 01JJY2099).

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An amperometric biosensor has been developed for the determination of H2O2 in plant samples. Horseradish peroxidase (HRP) is immobilized on a sandwiched nano-Au particle / m-phenylenediamine polymer film by glutaraldehyde cross-linking. The film is formulated on the carbon paste electrode (CPE) blended with ferrocene as an electron transfer mediator. On the low concentration range, the current response is related to the H 2O2 concentration linearly from 0 to 8×10 -6 M with a detection limit of 1.3×10-7 M. On a wider concentration range of 8×...