A novel, simple, rapid and highly sensitive assay and diagnostic tool for the burrowing nematode, Radopholus similis, was developed using a loop-mediated isothermal amplification (LAMP). The LAMP assay was targeted on the specific fragments of rRNA gene D2-D3 regions of R. similis. The detection limitation of the LAMP assay was as low as ten copies of plasmid DNA containing the target DNA, 10 fg of genomic DNA and 5 x 10(-5) nematodes. The detection sensitivity of the LAMP method for R. similis DNA was 10-100 times higher than normal PCR-based detection methods. The LAMP amplifications could b...
