In this study, we used native gradient-polyacrylamide gel electrophoresis and electroelution (NGGEE) to purify enzymatic proteins from Trichoderma koningii AS3.2774. With this method, we purified eight enzymatic proteins and classified them to the cellulase system by comparing secretions of T. koningii in inductive medium and in repressive medium. It resulted in 24-fold beta-glucosidase (BG) purification with a recovery rate of 5.5%, and a specific activity of 994.6 IU mg(-1) protein. The final yield of BG reached 8 mu g under purifying procedure of NGGEE We also identified BG using the enzyme...
