期刊论文

Gai, Hongwei

英文

Sensors and Actuators B-Chemical

0925-4005

2015

207

Part A

308-312

10.1016/j.snb.2014.10.077

Natural Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [21075033, 31301484]; State Key Laboratory of Analytical Chemistry for Life Science [SKLACLS1313]; Qinglan Project of Jiangsu province; Fund for Graduate Students of Jiangsu Normal University [2013YZD014]; Youth Foundation of Hunan Agricultural University [12YJ09]; Priority Academic Program Development of Jiangsu Higher Education Institutions

本校为第一机构

Super-resolution imaging at single-molecule level distinguishes each molecule within a diffraction-limited volume and precisely localizes its centroids. The foregoing can be achieved by monitoring the spectral blue shift of each quantum dot (QD) with a less than 50 nm-long DNA ruler under a spectral imaging microscope. The presence of two QDs can be revealed based on the fact that two similar-sized (color) QDs asynchronously blue shift and randomly blink. The centroid of a QD is easily simulated when only one QD is "on." After the centroids of both QDs are fitted, the distance between the two ...