Electrophoretic detection of genetic variability among Schistosoma japonicum isolates by sequence-related amplified polymorphism

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成果类型：

期刊论文

作者：

Song, Hui-Qun;Mo, Xi-Hao;Zhao, Guang-Hui;Li, Juan;Zou, Feng-Cai;...

通讯作者：

Zhu, Xing-Quan

作者机构：

[Song, Hui-Qun; Mo, Xi-Hao; Zhao, Guang-Hui; Li, Juan; Zhu, Xing-Quan] CAAS, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou, Gansu, Peoples R China.

[Song, Hui-Qun; Mo, Xi-Hao; Lin, Rui-Qing; Li, Juan; Weng, Ya-Biao] S China Agr Univ, Coll Vet Med, Guangzhou, Guangdong, Peoples R China.

[Zhao, Guang-Hui] NW A & F Univ, Coll Vet Med, Yangling, Shaanxi Prov, Peoples R China.

[Zhu, Xing-Quan; Zou, Feng-Cai] Yunnan Agr Univ, Coll Anim Sci & Technol, Kunming, Yunnan Province, Peoples R China.

[Liu, Wei] Hunan Agr Univ, Coll Vet Med, Changsha, Hunan Province, Peoples R China.

通讯机构：

[Zhu, Xing-Quan] C

CAAS, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, 1 Xujiaping, Lanzhou, Gansu, Peoples R China.

语种：

英文

关键词：

Genetic variability;Schistosoma japonicum;Schistosomiasis;Sequence-related amplified polymorphism

期刊：

ELECTROPHORESIS

ISSN：

0173-0835

年：

2011

卷：

期：

页码：

1364-1370

DOI：

10.1002/elps.201000600

基金类别：

National Basic Research Program (973 program) of ChinaNational Basic Research Program of China [2007CB513104]; Yunnan Provincial Program for Introducing High-level Scientists [2009CI125]; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30960280]; Natural Science Foundation of Yunnan ProvinceNatural Science Foundation of Yunnan Province [2007C206M]; Northwest A F University; Department of Science and Technology, Hunan ProvinceDepartment of Science & Technology (India) [2009FJ302]

机构署名：

本校为其他机构

院系归属：

动物医学院

摘要：

In the present study, sequence-related amplification polymorphism (SRAP) was utilized to study the genetic variability among Schistosoma japonicum isolates from different provinces in China, using Schistosoma mansoni from Puerto Rico for comparison. Five out of ten tested SRAP primer combinations displayed significant polymorphisms among S. japonicum isolates from China, namely ME2/EM1, ME4/EM1, ME4/EM6, ME5/EM4 and ME5/EM5. Analysis of the 61 S. japonicum samples from China with five SRAP primer combinations identified a total of 83 reproducib...

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Electrophoretic detection of genetic variability among Schistosoma japonicum isolates by sequence-related amplified polymorphism

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