期刊论文

中文

湖北农业科学

0439-8114

2010

49

9

2058-2060

10.14088/j.cnki.issn0439-8114.2010.09.001

国家自然科学基金资助项目（30800823）；

本校为其他机构

根据miR-15a成熟体序列合成1对miRNA寡聚单链DNA,克隆到真核表达载体pcDNA^TM6.2-GW/EmGFP-miR中,构建pcDNATM6.2-GW/EmGFP-miR-15a真核表达载体。瞬时转染肺腺癌A549细胞后,经Real-time PCR证明pcDNA^TM6.2-GW/EmGFP-miR-15a能有效表达miR-15a。试验结果为进一步研究miR-15a的功能和靶基因鉴定打下了基础。

A pair of oligodeoxynucleotides was synthesized according to the mature sequence of miR-15a,and cloned into pcDNA^TM6.2-GW/EmGFP-miR vector to construct pcDNA^TM6.2-GW/EmGFP-miR-15a.Then the recombinant vector was transfected into lung adenocarcinoma A549 cells,the expression of miR-15a was identified by real-time PCR.The results were helpful to further investigate the biological f...