Assembly of ascovirus HvAV-3h long DNA fragment using the Transformation-Associated Recombination (TAR) approach in yeast cells

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成果类型：

期刊论文

作者：

Heba A. H. Zaghloul;Zhengkun Xiao;Hengrui Hu;Guo-Hua Huang

通讯作者：

Huang, Guo-Hua

作者机构：

[Heba A. H. Zaghloul; Zhengkun Xiao] Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, PR China

[Heba A. H. Zaghloul] Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, 21511, Egypt

[Zhengkun Xiao] Yuelushan Laboratory, Hunan Agricultural University, Changsha, Hunan, 410128, PR China

[Hengrui Hu] State Key Laboratory of Virology, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

[Guo-Hua Huang] Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, PR China. ghhuang@hunau.edu.cn

通讯机构：

[Huang, Guo-Hua] H

Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, PR China.

Yuelushan Laboratory, Hunan Agricultural University, Changsha, Hunan, 410128, PR China.

语种：

英文

关键词：

Synthetic biology;Ascoviruses;Transformation-Associated Recombination (TAR);Yeast;HvAV-3h;BAC/YAC shuttle vector

期刊：

BMC Biotechnology

ISSN：

1472-6750

年：

2025

卷：

期：

页码：

1-13

DOI：

10.1186/s12896-025-00964-8

基金类别：

This work was funded by the National Natural Science Foundation of China (grant number 32250410311) provided to Heba A. H. Zaghloul and China Agriculture Research System (grant number CARS-23-C08) provided to Guo-Hua Huang.

机构署名：

本校为第一且通讯机构

院系归属：

植物保护学院

摘要：

Synthetic biology is a young but rapidly growing field that allows for assembling long DNA fragments, including complete chromosomes. A key approach for long-DNA assembly is the Transformation Associated Recombination (TAR), which relies on efficient homologous recombination in yeast cells. Recent reports indicate that the TAR method efficiently assembles some human and animal viruses characterized by their large DNA genome size. The application of the TAR method to synthesize long DNA fragments derived from insect viruses is scarce. Therefore,...

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Assembly of ascovirus HvAV-3h long DNA fragment using the Transformation-Associated Recombination (TAR) approach in yeast cells

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