期刊论文

中文

园艺学报

0513-353X

2006

33

1

130-133

10.16420/j.issn.0513-353x.2006.01.029

国家转基因专项资助项目（YJ04-B-02） 湖南农业大学***基金项目（03YJ09）

本校为第一机构

对转rol基因枳橙B、D、E3个系及对照田间高接植株进行了PCR分子鉴定、RT-PCR转基因表达分析和生物学特征的观测。PCR分析表明rolA、rolB、roIC已成功转入到3个转化枳橙系中。利用RT-PCR在田间转化植株中只检测到npt Ⅱ和rolC基因的表达，而rolA、rolB基因未检测到。3个转化系枳橙均表现出顶端优势减弱，侧枝增多，植株矮化性状明显，高度只有对照植株的44％～50％，节间长度缩短，叶面积减少；光合作用增强；内源赤霉素、生长素、玉米素等促进类激素在中部、下部叶片含量均高于对照，这可能是诱导上述形态特征的变化的因素之一。

Molecular identification, physiology evaluation and morphological observation of the rolA, rolB and rolC transgenic citrange clones （B, D, E） were conducted. PCR analysis indicated that the rolA, rolB, rolC genes were integrated into the citrange genome. The expression of the transgenes npt Ⅱ and rolC was detected by using RT-PCR; while the transgenes rolA and rolB did not show any RT-PCR product, and it was not clear for the factors related to their expression. In comparison with the control plants, the rol transgenic plant showed evident d...