期刊论文

中文

园艺学报

0513-353X

2006

33

4

842-844

10.16420/j.issn.0513-353x.2006.04.032

江西省科技厅重点科研资助项目（963007）；

本校为其他机构

以中华猕猴桃矮型种质为离体培养材料,建立试管无性系,对其离体茎尖的玻璃化法超低温保存技术进行研究,探讨猕猴桃种质长期保存的适宜途径.结果表明,含1～2个叶原基(1.5～2.5 mm)的茎尖,在5%二甲基亚砜(DMSO)+5%蔗糖+MS培养基上预培养4 d后,于室温下用2 mol/L甘油+0.4 mol/L蔗糖预处理30 min,再在0℃下用玻璃化液(PVS2)脱水处理40 min并迅速投入液氮中,保存24 h后接种至继代培养基上再培养,成活率和再生率分别为56.7%和51.6%.再生植株生根后可移栽成活.

Experiments were conducted to provide an appropriate means for long-term preservation of kiwifruit germplasm using cryopreservation by vitrification. Shoot-tips of a dwarf genotype of kiwifruit were cultured in vitro to obtain aseptic clones. The results showed that shoot-tips 1.5 -2.5 mm in length with one or two leaf primordium were precuhured in 5% DMSO ＋5% sucrose ＋ MS medium for 4 days. Pretreated shoottips with 2 mol/L glycerol ＋ 0. 4 mol/L sucrose for 30 min at room temperature, followed by dehydration with PVS2 （30% glycerol ＋ 15% ...