期刊论文

中文

园艺学报

0513-353X

2008

35

9

1393-1396

10.16420/j.issn.0513-353x.2008.09.003

(2005DA531):国家科技部'十五'攻关项目 (62020206003):湖南农业大学基础基金项目

本校为第一机构

应用微波辐射技术,用乙醇—乙酸双溶剂法从‘中薯3号’马铃薯薯皮中提取了马铃薯植株和块茎中的生物碱龙葵素,对所得初提物进行了纯化,并应用HPLC技术对提取的龙葵素进行了定量分析。通过4因素3水平正交试验确定了本法的最佳提取参数。结果表明微波辐射提高了提取效率并缩短了提取时间。正交试验所得最佳提取流程是：将样品和乙醇—冰乙酸（体积比为100∶10）按10（g）∶200（mL）的比例混合,在540W的微波功率下提取6min。

Solanine was extracted from peel of a potato variety ＇ Zhong Shu 3 ＇ by using microwave and ethanol/acetic acid solvent. Crude extract was further purified and then qualified by HPLC. The optimized extraction parameters were obtained through a 4 factor, 3 level orthogonal experiment. The results showed that the use of microwave not only increased the extraction efficiency but also reduced the extraction time. The best extraction procedure obtained from the orthogonal experiment was first to mix peel powder with ethanol/acetic acid （100/10, V...