期刊论文

Sterility Characteristics and the Genetic Law of a Reverse Thermosensitive Genic Male Sterile Mutant E6421S in Peppers

Liu, F.

中文

园艺学报

0513-353X

2019

46

6

1112-1122

10.16420/j.issn.0513-353x.2018-0900

国家重点研发计划项目(2017YFD0101906).

本校为第一机构

对辣椒反向温敏雄性核不育突变材料E6421S的育性转换临界温度、生理生化特点及遗传规律进行了研究。结果表明:E6421S育性转换临界温度为28℃,28℃以上表现为可育,28℃以下不育;25℃下显微观察发现E6421S绒毡层在四分体时期与花粉母细胞黏连,导致花粉母细胞发生败育;生理生化分析发现E6421S花蕾各发育时期可溶性蛋白含量及过氧化氢酶活性明显低于正常育性的原品种,而游离脯氨酸含量与正常育性的原品种相反,随着花蕾的发育呈下降趋势;与正常育性的原品种6421杂交后代群体性状调查表明,E6421S不育性状由隐性单基因控制。

The critical fertility transition temperature, physiological and biochemical characteristics and genetic law of a reverse thermo-sensitive male sterile mutant E6421S in peppers were studied. The results showed that the critical fertility transition temperature of E6421S was 28 ℃. It was fertile above 28 ℃，and was sterile below 28 ℃. Microscopic observation in 25 ℃ showed that the tapetum of E6421S adhered to pollen mother cells at tetrad stage，leading to the abortion of pollen mother cells. Physiological and biochemical analysis showed th...