期刊论文

Identification and Expression Profiling Analysis of TCP Family Genes Involved in Growth and Development in Camellia sinensis

Huang, J.

中文

园艺学报

0513-353X

2019

46

12

2369-2382

10.16420/j.issn.0513-353x.2019-0320

31670691:国家自然科学基金 31500567:国家自然科学基金 31470692:国家自然科学基金

本校为第一且通讯机构

基于茶树基因组数据,通过生物信息学方法,对茶树全基因组TCP家族成员进行染色体定位、基因结构、编码蛋白特性和系统进化关系分析,同时采用荧光定量PCR验证茶树TCPs在不同组织部位的相对表达水平。结果表明,茶树全基因组中共鉴定出36个具有典型TCP保守结构域的转录因子基因,其CDS全长和编码氨基酸的数量分别在453 ~ 1 824 bp和150 ~ 607 aa之间,命名为CsTCP1 ~ CsTCP30。系统进化分析显示,茶树TCP转录因子家族可分为2个亚族:Class I和Class II,并可进一步分为8个亚组(A ~ H),其中Class I亚族中的TCP蛋白属于PCF或TCP-P类型,而Class II可分为CYC/TB1和CIN两种类型。基因结构和保守结构域分析结果...

In this study, a genome-wide analysis of TCP genes was performed in Camellia sinensis, including identification, chromosomal localization, gene structure, phylogenetic analyses and expression analysis in different organs. In all, thirty-six proteins were identified with typical TCP domains. The length of CDS is from 453 to 1 824 bp, and proteins' length is from 150 to 607 aa (amino acids) .These identified CsTCP members were named as CsTCP1-CsTCP30. Phylogenetic and comprehensive bioinformatics analyses of these genes revealed that Camellia sin...