期刊论文

Identification and Expression Analysis of the KCS Gene Family in Pepper

Zou, X.

中文

园艺学报

0513-353X

2020

47

2

370-380

10.16420/j.issn.0513-353x.2019-0574

湖南省重点研发计划项目（2017NK2373）；

本校为其他机构

为深入了解β-酮脂酰辅酶A合酶(β-ketoacyl-CoA synthase,KCS)基因家族在辣椒基因组中的特征,利用生物信息学方法鉴定所有KCS基因家族成员,对基因染色体定位、系统发育关系、基因结构、蛋白保守基序和组织表达模式等进行分析,并通过qRT-PCR方法检测基因在高温、低温和NaCl胁迫下的响应。从辣椒基因组中共鉴定出22个KCS家族基因,其不均匀地分布在辣椒的9条染色体上;系统发育分析表明辣椒KCS基因家族可分为4组,每组含有不同数量的基因;该家族成员含有0 ~ 3个内含子,保守基序有6 ~ 14个;KCS家族基因具有不同的表达模式;高温、低温和NaCl处理可以抑制或激活该家族成员的表达。

In order to understand the characteristics of the β-ketoacyl-CoA synthase(KCS)gene family in the Capsicum annuum genome deeply,all KCS gene family members were identified by using the bioinformatics methods,hereafter,the genes chromosomal location,phylogenetic relationship,gene structure,protein conserved motifs,tissue expression pattern and response to high temperature,low temperature and NaCl stress were analyzed. The results showed that a total of 22 KCS genes were identified and distributed on 9 chromosomes. Phylogenetic analysis indicated...