期刊论文

Song, Yong;Wang, Xiaowu

英文

园艺学报(英文版)

2095-9885

2021

7

1

31-37

10.1016/j.hpj.2020.05.003

supported by the National Natural Science Foundation of China （Grant No. 31630068）； the National Program on Key Research Project （Grant No. 2016YFD0100307）； the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences； the Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, China；

本校为第一且通讯机构

Brassica rapa is one of the most important leafy vegetable crops with large cultivated area in China. To increase the availability of DNA markers in B. rapa, we developed insertion-deletion（In Del） markers utilizing high-resolution melting（HRM） curve analysis. We designed primers for 252 In Dels（≥ 3 bp） evenly distributed in the genome and tested gene polymorphisms with eight accessions. In total, 208 markers were speciically ampliied, and 148 In Dels with polymorphism were genotyped successfully using HRM. We further analyzed the correl...