期刊论文

Ma, X.

中文

园艺学报

0513-353X

2022

49

6

1247-1260

10.16420/j.issn.0513-353x.2021-0323

国家重点研发计划项目（2018YFD10003000）； 湖南省自然科学基金项目（2020JJ4040）；

本校为第一且通讯机构

柑橘溃疡病菌(Xanthomonas citri subsp. citri,Xcc)侵染感病种质冰糖橙叶片后,在侵染部位形成典型的火山口凸起症状,但在抗病种质枸橼C-05叶片侵染部位呈现逐渐褐色坏死,未形成溃疡病典型症状。基于前期冰糖橙和枸橼C-05叶片接种Xcc的转录组测序结果,分析鉴定的20个甜橙SWEET受Xcc诱导表达变化,结果显示SWEET2a和SWEET17d在冰糖橙中受Xcc诱导上调表达,SWEET12b在枸橼C-05中受Xcc诱导上调表达;实时荧光定量PCR验证,SWEET2a在冰糖橙叶片中受Xcc诱导高表达而在枸橼C-05叶片中表达变化不显著;SWEET2a在不同柑橘种质同源基因编码的氨基酸序列相似性为94.6%,其启动子顺式作用元件在冰糖橙和枸橼C-05中...

After the infection of citrus canker pathogen Xanthomonas citri subsp. citri （Xcc），infected the leaves of the susceptible germplasm'Bingtang'Sweet Orange，it formed typical crater bulge symptoms at the infected site，but gradually showed brown necrosis at the infected site of the leaves of the resistant germplasm Citron C-05，without typical symptoms of canker disease. Based on the transcriptome sequencing analysis of'Bingtang'Sweet Orange and Citron C-05. leaves inoculated with Xcc，the expression of 20 CsSWEET induced by Xcc was compared. ...