期刊论文

Ma, X.

中文

园艺学报

0513-353X

2022

49

6

1213-1232

10.16420/j.issn.0513-353x.2021-0331

31720103915:国家自然科学基金 2020JJ4040:湖南省自然科学基金 2019RS1052:湖南创新型省份建设专项

本校为第一且通讯机构

为探究HSP20在柑橘(Citrus L.)响应溃疡病菌(Xanthomonas citri subsp. citri,Xcc)侵染过程中发挥的作用,鉴定CsHSP20家族基因。生物学信息分析显示共有42个,分为11个亚族,分布在9条染色体上,编码135 ~ 373个氨基酸,蛋白分子量15.17 ~ 41.71 kD,等电点4.53 ~ 10.07,92.9%的基因没有或只有1个内含子,各亚族基因间有高度相似的结构和保守基序,每个基因的启动子都具有与激素或胁迫相关的顺式作用元件。其次,对感病的冰糖橙和抗病的枸橼C-05进行接种Xcc和40 ℃热胁迫处理,qRT-PCR结果显示CsHSP20家族中的HSP17.9、HSP23.3、HSP18.5和HSP18.0均上调表达,但抗感材料之间差异明显,HSP23.3在枸橼C-05中受...

Citrus canker，caused by is a bacterial disease，which severely influence citrus industry，and its pathogenicity is significantly enhanced under higher temperature and humidity environmental condition. HSP20 genes are abundant and highly conserved in higher plants，they play vital defense roles when plants are under biotic or abiotic stress. In order to investigate the role of HSP20 genes in response to Xanthomonas citri subsp. citri infection，the genes of HSP20 family in Citrus sinensis were identified. The analysis of biological information ...