期刊论文

中文

园艺学报

0513-353X

2025

52

04

1093-1104

10.16420/j.issn.0513-353x.2025-0368

国家自然科学基金项目(32441073,32320103011)；

本校为第一且通讯机构

以新型可视化报告系统RUBY为标记,筛选获得基因递送高效辣椒受体材料3份,并结合促再生因子小肽CaREF1,构建了辣椒高效遗传转化体系。结果表明,线椒‘L27’、朝天椒‘遵辣1号’及珠子椒‘1-51’的基因瞬时表达效率显著高于其他材料;利用搭载RUBY报告系统的发根农杆菌K599进行遗传转化,于不定芽再生分化培养基(MS+5 mg·L-1 6-BA+1.2 mg·L-1 IAA+10 nmol·L-1 CaREF1)及壮芽培养基[MS+（5～10）mg·L-1 6-BA+0.5 mg·L-1 IAA+3 mg·L-1 GA3+10 nmol·L-1 CaREF1]培养60 d后,平均再生效率可达84%±7%,平均再生芽数约为5.52个,阳性转化率约为5‰。基于该体系建立了辣椒农杆菌介导的可视化遗传转化体...

Using the novel visual reporter system RUBY as a screening marker, combined with the regeneration-promoting peptide CaREF1, a pepper genetic transformation system were constructed and identified three efficient recipient materials for gene delivery. The transient expression efficiency of RUBY was significantly higher in the pepper genotypes'L27' (line pepper), 'Zunla-1' (chili pepper), and '1-51' (bead pepper) compared to other materials. Agrobacterium rhizogenes K599, carrying the pKSE401-RUBY vector, was used for transformation. After 60 days...