期刊论文

The Sequence and Function Analysis of AVR-Pita Gene Family of Rice Blast Fungus, Magnaporthe oryzae in Philippines

中文

中国生物防治学报

2095-039X

2018

34

3

488-498

10.16409/j.cnki.2095-039x.2018.03.006

湖南省自然科学基金

本校为第一机构

根据已克隆的AVR-Pita及其同源基因序列设计4对引物,对来自菲律宾的74个稻瘟病单孢菌株DNA进行PCR扩增和序列分析,研究了与水稻抗病基因Pi-ta互作的稻瘟病菌无毒基因AVR-Pita及其同源基因序列多态性与进化关系。结果显示,在42个菌株中扩增出AVR-Pita1基因目的条带,31个菌株中扩增出AVR-Pita3基因目的条带,55个菌株中扩增出AVR-Pita4基因目的条带,但所有供试菌株均未扩增出AVR-Pita2基因目的条带。对PCR产物进行测序分析,发现AVR-Pita1基因在菲律宾的生理小种中存在序列多态性,可划分为5个单倍型,共有10个多态性位点;AVR-Pita3和AVR-Pita4基因序列较保守,序列比对后没有发现多态性。对不同单基因...

For controlling rice blast and providing theory for breeding and selecting resistant rice variety, we studied the sequence polymorphism and function of avirulence gene AVR-Pita homologs. According to the cloned AVR-Pita homologs, we designed 4 primer pairs, selected 74 isolates from Philippine and distracted their DNA as template for PCR. The results showed that 42 isolates had the band of AVR-Pita1 gene, 31 isolates had the band of AVR-Pita3 gene, and 55 isolates had the band of AVR-Pita4 gene. However, no isolates showed the band of AVR-Pita2 gene. Sequences analysis of PCR products showed t...